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.Abbreviations used: CD, circular dichroism; DTNB, 5,5'-dithiobis-(2-nitrobenzoic) acid; EDTA, ethylenediaminetetracetic acid; GdnHCl, guanidine hydrochloride; GR, yeast glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; HEPES, n-(2-hydroxyethyl) piperazine-n'-(2-ethanesulfonic acid); HPLC, high performance liquid chromatography; MG, molten globule
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. Thus the β-sheet on the surface of the catalytic domain, Which is exposed to water in native ACYI, might unfold first
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.0 do not support this explanation (Fig
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.Membrane binding for PLCβ was determined by following the increase in acceptor fluorescence from PLCβ Trp donors to model membranes doped with 0
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