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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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. This is called "wraparound" binding because the two domains in CaM wrap around the α-helical target (8,9)
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. The same figure also shows the S^sup 2^^sub f^ contours when an additional nanosecond-timescale internal motion is present with time constant τ^sub is^ = 2 ns and order parameter S^sup 2^^sub s^ = 0
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. The preparation contained approximately equal amounts of BLG A (mass 18365) and BLG B (mass 18278)
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