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. Ligand-protein contacts (LPC) was derived with LPC software: automated analysis of interatomic contacts in proteins
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. Specifically, we believe that ensembles for proteins obtained in the EV limit (48,124-127), will have characteristics that match predictions from field theories
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. At the other end of the spectrum, recent experimental work and some modeling efforts suggest that a new paradigm is in order for denatured-state ensembles (100,101,130)
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. J Exp Med 2000; 191:515-528(18) Okazawa H, Motegi S, Ohyama N, et al
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. RESULTS AND DISCUSSION Thermal and GndHCl-induced unfolded states All α-amylases investigated in this study consist of a monomeric structure with three domains
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. Our gradient coil capacity allows still higher gradient strengths, but these cause larger eddy currents and possibly also signal distortions in MRS experiments
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. The C^sub p^(T) data indicate a gradual reduction in the vesicle population and a concomitant increase in the disks population during incubation (Fig
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. Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure
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.5 kbar (data not shown), a pressure at which a large number of protein dimers and higher oligomers have been found to undergo subunit dissociation (Gross and Jaenicke, 1994; Heremans and Smeller, 1998)
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. Similar results were obtained for all other two-state protein sequences shown in Table 1
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.Osmotic stress techniqueThe dependence of the osmotic pressure of BLG solutions on protein concentration, pH, and added NaCl concentration was determined using an osmotic stress technique
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.Samples with fluorophore concentrations of 5 µM were made in different concentrations of glycerol (0%-82
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