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. For each of the amino acid sequences shown in Table 1, ensemble averages and conformalional distributions were obtained from an ensemble with a sample size of 10^sup 4^ and the ensembles were generated as discussed above
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. Based on our observation that fertilized X
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. Moreover, the quasi-docked species observed in our study appears to form very rapidly, with a rate constant that is two to four orders of magnitude faster than the value reported for formation of the intermediate for the immobilized 4WJ ribozyme under similar conditions (Tan et al
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. Hence, quasi-docked and specifically-docked ribozyme species are readily distinguished in our diffusion spFRET experiments
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. Clearly, the four-way helical junction in the native ribozyme is the most important determinant for proper folding, guiding the approach of arms A and B
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.* Point Count--The net value of the card count at the end of a hand
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