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Native proteins are surface-molten solids: application of the Lindemann criterion for the solid versus liquid state
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It can be concluded, then, that EI^sup sc^ at low pH unfolded via a moltenglobule-like species, as it has been described in other large multimeric proteins (49)
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Indeed, if one compares liquid and frozen state profiles at the same effective ANS concentration in the liquid phase (bulk solution versus liquid water pools formed at intergranular spaces), we see that strong ANS binding is largely abolished in ice
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Comparison of solute-induced protein stabilization in aqueous solution and in the frozen and dried states
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The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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Age at 50% maturity was calculated by using our estimate of length at 50% maturity and a VBGF rearranged to the form[t
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8 shows a comparison of the peak positions for the SL and WT versions of the ribozymes
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However, it was also found that if the cells in monolayer were cultured in medium supplemented with FCS for one further passage then the cells would subsequently form pellets in the DMEM/10%FCS medium
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Many of its pharmacists have already received additional training and certification to help their patients manage diabetes
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. Thermodynamic data on the conformational stability of proteins can be obtained using several chemical or physical perturbants, including chaotropic agents (guanidine hydrochloride, urea), hydrostatic pressure, and temperature
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. According to the definitions mentioned above, such folding intermediates of GR can possibly be described as MG states
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. the tertiary structure of EI^sup sc^ remained unaltered, as suggested by fluorescence and ANS-binding (Fig
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. Additionally, the scan-angle was changed in cases of repeated scans
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.Looked at another way, our structure is a more detailed examination of the sort of dynamic processes that must be occurring within the "molten globule" phase of protein folding
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. The Colorocs toner has melted and coated individual fibers; no toner film is visible in the SEM, while the solvent dye is clearly present on the fibers, as seen in the OM picture (reproduced here in black and white; the dye is a red color and can be clearly seen in the color originals)
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.During the in vitro monolayer expansion, the cells typically dedifferentiate to a fibroblastic morphology and phenotype
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