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The activity decreased rapidly at higher temperatures (>65°C) due to inactivation of the enzyme
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We have developed a method for analyzing ^sup 15^N-spin relaxation measured at two magnetic fields that corrects (τ^sup B^^sub m^)^sub app^ for internal motion up to ~4 ns, giving (τ^sup o^^sub m^)^sub app-corr^ (22)
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CPM-PLCβ is sensitive to binding by Gβγ-subunits giving an ~200 cm^sup -1^ shift in emission energy and an ~2-fold increase in intensity (G
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Relaxation functions in folding kineticsWe first take a very simple case: if the nonnative gutter-stabilizing interactions are perfectly balanced with the entropy changes at each stage of the dimension reduction, then the free-energy profile is itself flat, and the d diffusive dimensions form an effective one-dimensional path along which the folding takes place
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In the diffusion spFRET measurements described in this article, by labeling the ends of the loop-bearing arms with donor and acceptor dyes, we use the FRET efficiency E as an indicator of the distance between arms A and B of the ribozyme during the docking process
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