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. 1 B shows ^sup 1^H MR spectra obtained from these voxels, exhibiting notable differences: In the nucleus, the nonwater signal was very small, whereas large peaks typical of triglycerides could be observed in the animal and vegetal cytoplasm-they represent methine, the glycerol backbone, methylene, and methyl residues, as indicated in the figure
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. The qualitative structural implication is, therefore, that the second CaM monomer in the dimeric complex is placed along this axis in such a way that the tips of the N- and C-terminal domains touch each other
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. FORMATION OF iPP TONERSWe selected to produce the IPP toners for the initial tests because Lloyd [10, 11] had previously produced IPP film containing spherical particles using TIPS
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. The activity decreased rapidly at higher temperatures (>65°C) due to inactivation of the enzyme
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. In a previous study, we probed the role of the two proximal GlcNAc residues in tethering the glycans attached to one subunit to the side chains of amino acid residues of an adjacent subunit at the noncanonical interface (18)
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. We do not, therefore, see a fundamental contradiction between our experimental results and the MD simulations
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.Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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.Amyloidogenic Self-Assembly of Insulin Aggregates Probed by High Resolution Atomic Force MicroscopyJansen, RalfABSTRACTAs the application of high-resolution atomic force microscopy (AFM) has led us recently to the discovery of a unique pressure-induced circular amyloid, we used the same approach to examine morphological events accompanying insulin aggregation under ambient conditions
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