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The Φ angles, recalculated with the smaller anisotropy, were essentially within the error margins of the earlier estimates black gambling jack rule .
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Sample solutionsSolutions for microscopic observations were prepared by mixing PEG 3000 or PEG 10000 in twice-distilled water until dissolution, adding T4 DNA with further mixing and incubation for 30 min, adding DAPI with further mixing and incubation for 30 min, and finally adding alkali chloride solution and letting the sample solution sit for 1-2 h before observations black gambling jack rule .
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