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The final concentrations were 5 mM MgCl^sub 2^ and 2
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the accessibility of atoms and residues, the backbone dihedral angles, and the hydrogen bond and electrostatic networks of the protein
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3 A compares the effect of 2 µM BSA on the ANS intensity in supercooled liquid and frozen states at -5°C
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A rough estimate of the minimum number of binding sites, as done above with azurin, yields n ~20, which is four times larger than the corresponding value in liquid solution (5)
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