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5 c, and as known for all α-amylases studied here, the protein concentration has no effect on the transition temperatures (17)
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The measurement was repeated on five individual oocytes
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MATERIALS AND METHODSEnzyme purification and characterizationHuBChE was purified to homogeneity from outdated human plasma according to an improved protocol based on that of Grunwald et al
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The predicted dependence of η^sub r^ on mass fraction for particles with a v of 3
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Protein transfer between A-I-containing lipoprotein subpopulations: evidence of non-transferable A-I in particles with A-II
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Linked folding and anion binding of the bacillus subtilis ribonuclease P protein
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Manuscript approved for publication 16 September 2004 by the Scientific Editor
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This finding is compatible with the possibility that the eggs were P
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- As previously pointed out (Rietveld and Ferreira, 1996; 1998; Ferreira and De Felice, 2001), dimerization may lead to a significant increase in stability against chemical modification of GR, including thiol oxidation, de-amidation, or proteolytic digestion
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. These results suggested that during ACYI thermal denaturation, the dominant events were the unfolding of the β-sheet (1638 cm^sup -1^) and the formation of the intermolecular β-sheet in aggregates (1617 cm^sup -1^)
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. Then, one is tempted to ask, is there any relationship among the different functions and structures of the PTS proteins on one side, and the thennodynamic parameters and folding properties on the other? To answer this question, it is necessary to describe the conformational preferences of proteins, where studies of different members of the family have been described
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.The peak position for the SL 3WJ remains in the low-FRET region of the histograms, independent of the Mg^sup 2+^ concentration, indicating that this ribozyme construct is incapable of folding to a compact structure (Fig
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