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At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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Massey and Williams (1965) found 3
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As the temperature was increased, the intensity of the hands from the native secondary structures decreased, and two new bands (1618 and 1682 cm^sup -1^) appeared at temperatures above 70°C
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ANS binding experiments, extended to a set of proteins including serum albumin, α-amylase, β-galactosidase, alcohol dehydrogenase from horse liver, alcohol dehydrogenase from yeast, lactic dehydrogenase, and aldolase, confirmed that a stressed condition of the native fold in the frozen state appears to be general to most proteins and pointed out that oligomers tend to be more labile than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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The measured weights were taken as their dry weights (h = 0 g water/g dry powder, denoted by g/g in the following)
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The complex properties of protein aggregation and amyloid formation require development of sophisticated although simple techniques which can provide detection as well as direct output of structural changes of protein assemblies, e
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Human carbonic anhydrase II is considerably expanded by the interaction of GroEL
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4 a, the helical groove-depth is 1
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. The three cubes in the image indicate the actual position and width selected for the recording of localized spectra from isotropic voxels of (180 µm)^sup 3^ in each compartment
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. In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig
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. Data fitting suggests that, in contrast to the double-exponential kinetics in the first jump (τ^sub fast^ = 190 s, τ^sub slow^ = 1630 s), the data in the second jump are well approximated by a single exponent with a relaxation time τ = 190 s, which is similar to τ^sub fast^
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