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The order of events could be characterized as the intensity decrease of the band at 1629 cm^sup -1^ > the intensity increase of the band at 1617 cm^sup -1^ > the intensity decrease of the band at 1649 and 1658 cm^sup -1^
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Dynamic light-scattering studies The dynamic light scattering measurements were done using a DynaPro-MS800 dynamic light scattering instrument (Protein Solutions, Lakewood, NJ)
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Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24)
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How important is the S-S bond for folding and stability? Eur
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The RT^sup n^^sub 1^ values for the CaM:SEF2-1mp complex are superimposed onto the S^sup 2^ contours in Fig
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If the interface has void volume and the protein cannot easily deform, then water will instead fill the voids and promote membrane disassociation (see discussion in Teng and Scarlata, 1993)
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Interestingly, the activation energy E^sub a^ = 60 ± 8 kcal/mol of the slow heat-unfolding phase in apoA-2:DMPC disks (Fig
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The advantage of the hypergutter approach, however, is that it identifies diffusive subspaces in cases where the standard diffusion-collision model does not
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© 2005 by the Biophysical Society0006-3495/05/01/172/12 $2
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The "Species" column show the categories assigned inthe survey reports
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In other words, the second MD trajectory segment covers a small subspace of the conformational space sampled by the first segment and hence reduces the overall size of the elements of the covariance matrix σ through averaging over the combined trajectories
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We found that all three constructs were active in cleavage, and that the cleavage half-lives were 21
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