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Effect of modulating unfolded state structure on the folding kinetics of the villin headpiece subdomain
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This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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This trend is consistent with a pronounced binding capacity of oligomers whose subunits tend to dissociate in frozen aqueous media thereby making accessible large hydrophobic patches of the subunit interface
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The spectrum of the slice through the nucleus hence contained cytoplasmic signal
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5-ms time bins that we have used for the FRET histogram data
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