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Modulation of protein structure and function by asparagines linked glycosylation
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Processes that disrupt or uncouple this balance have the potential to alter normal cell turnover, ultimately resulting in the induction of lung pathology and disease
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2 show that, relative to the WT, onset of ANS binding occurs at lower concentrations with the mutants and in the case of I7S and F110S saturation is not achieved up to 200 µm ANS
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In considering data obtained by x-ray crystallography, Frauenfelder and Petsko (11) found that binding of oxygen was accompanied by a reduction in B-factors of residues close to the heme pocket in myoglobin
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(15) and references therein), MD simulations (16), and incoherent neutron scattering (17)
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We did not observe either of these phenomena in our experiments
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As shown, regardless of the PEG length, the order of compaction potential for metal chlorides with the exception of LiCl is as follows:NaCl > KCl > RbCl > CsCl
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Diffusible, nonfibrillar ligands derived from Abeta1-42 are potent central nervous system neurotoxins
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These enzymes catalyze the hydrolysis of a minor component in membranes, PI(4,5)P^sub 2^, to generate two second messengers which lead to a rise in intracellular calcium and activation of protein kinase C (see Rebecchi and Pentyala, 2000; Rhee, 2001)
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Activation of PLCβ by G-protein subunits thus appears to occur through lateral association of the membrane-bound species
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As expected, the T^sub g^ of our EVA copolymers lies between the T^sub g^ values of the monomers (polyethylene -125°C, polyvinylacetate 31°C [3])
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The reactivity of the antibodies produced by the hybridomas was then determined
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Each standard concentration was used in triplicate
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These data support the proposition of Jakob et al16 that the conditions of expansion of human articular chondrocytes can modulate their ability to re-enter the differentiation programme when transferred to a three-dimensional environment
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. In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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.RESULTSCaM R^sub 1^, and R^sub 2^ relaxation rates were measured at 600 and 400 MHz ^sup 1^H frequency and the ^sup 15^N-{^sup 1^H} NOE data at 600 MHz
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. In contrast to the interior, pressure has little effect on the membrane surface charge (Scarlata and Rosenberg, 1990)
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. * Corresponding author - woodman
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