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Column calibration was done with a set of eight proteins of known molecular weights (thyroglobulin, 669 kDa; ferritin; 440 kDa; alcohol dehydrogenase, 150 kDa; bovine serum albumin, 66 kDa; ovalbumin, 45 kDa; carbonic anhydrase, 29 kDa; trypsinogen, 24 kDa; and cytochrome C, 12 kDa)
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Thus, both the appearance of tyrosine emission and the red-shift of tryptophan fluorescence indicated extensive unfolding of GR at 3 M GdnHCl
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The fluorescence spectral centers of mass showed a marked (~13-14 nm) red-shift between 1 M and 3 M GdnHCl (Fig
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Calorimetric measurements Calorimetric measurements were carried out using a Setaram Micro DSC III differential scanning calorimeter (DSC) with a 0
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ANS binding studies were done on rSBA
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The negative slope in the heating and cooling curves in Fig
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This may reflect the large structural change and the crossing of the free and bound buildup curves (Fig
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However, in general it was found that as the blocks progressed, there was an increase in the luck rating rather than a decrease, F(7, 98) = 2
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