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8), 150 mM sodium chloride, 15% glycerol, 1 mM Ca^sup 2+^/Mn^sup 2+^, and 200 mM lactose)
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8 kcal/mol for the second transition) and 51
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9%) than those at neutral pH (pH 7 (44
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To address these questions, we carried out calorimetric and thermal measurements by using DSC and several spectroscopic techniques (Table 3)
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Here the process seems to run the other way around
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Upon Ca^sup 2+^ binding, a conformational change enables it to interact with over 100 different target proteins, including transcription factors (for reviews, see Van Eldik and Watterson and others (6,7))
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Akke and co-workers (38) have also found larger ΔH^sup #^ values for residues that are part of regular secondary structure and smaller values for loop residues, in their study on the C-terminal domain of CaM
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Data from this work suggest that unbound ThT is also intrinsically quenched and functions similarly to a molecular rotor, which in combination with its environmental dependence provides a blue shift to the characteristic 482 nm wavelength when bound to amyloid fibrils
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High-resolution probing of local conformational changes in proteins by the use of multiple labeling: unfolding and selfassembly of human carbonic anhydrase II monitored by spin, fluorescent, and chemical reactivity probes
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In the triple-helix example that we looked at in detail, the nonnative hydrophobic strips that accelerate the final two-dimensional search are "activated" only when the helices themselves form, at least partially (by the formation of some native interactions) and when their axial rotational degrees of freedom align
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A generalized reaction field method for molecular-dynamics simulations
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Tissue sections were incubated in an antigen retrieval solution (citrate buffer) (BioTek Solutions, Santa Barbara, Calif) in a microwave oven and stained on a Tech Mate 1000 automated immunostainer using an avidin/biotin complex staining procedure (BioTek Solutions)
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1 M sodium cacodylate buffer at pH 7
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Type 2 RP was applied to the NSAIDs in November 1995
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This prior-authorization program is similar to RP in that Pharmacare will fully reimburse a drug only if it approves the drug's use
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Various other NSAIDs (1) were either delisted or required prior authorization at various times over the sample period, but these drugs collectively accounted for less than 7 percent of all NSAID prescribing in the 19 months before Type 2 RP
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In some cases, the product applicators did not have the proper child-resistant packaging, did not include the accompanying instruction leaflet required, or gave the animal weight in kilograms instead of pounds
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"I don't think it's going to evolve at a pace that might increase the number of available drugs for select populations because of the negative feedback that has arisen, particularly among constituents who currently control the purse strings for research
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There are the obvious connections: things like OTCs and health and beauty aids, stuff like cough-cold and pain relief, skin care, foot cafe and sun care
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. Interestingly enough, the addition of both effectors to EI^sup sc^ partially compensated for the stabilizing effect of Mg^sup 2+^
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. The secondary structure of EI^sup sc^ upon binding was not largely affected (Table 4), but there were subtle changes in the stability of the protein (Table 3)
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