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Based on mass spectromctric results, a structural model of ACYI was proposed, and it was suggested that each ACYI subunit might be composed of two domains with α/β folds: the catalytic domain and the dimerizalion domain (17)
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MATERIALS AND METHODS Materials All chemicals were of the highest purity grade available from commercial sources and were used without further purification
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The plasmid carrying the WT sequence was a generous gift from Prof
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Given δ^sub 1,2^ and a reasonable maximum of Δ^sub ex^ (~6 ppm), a correlation plot of R^sub ex^ vs
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Consequently, the on-rate of the equilibrium between free and CaM-bound peptide will be faster than diffusion controlled, which explains the fast exchange and the high affinity observed in the CaM:SEF2-1mp interaction (11)
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They showed that DNA compaction by both monocations is preferred at higher temperature; however, the slopes of these dependencies were different as a result of different thermodynamic parameters of DNA compaction
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The most important studies on DNA-monocation binding are summarized in Table 1, which shows the activity sequences, methods used in research, and the corresponding citations in our list of references
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This role is illustrated strikingly by the SL 4WJ, which can fold into a conformation resembling the docked structure even in the absence of loop A
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For this reason all cells were exposed to the DMEM/ITS+/TGF-[beta]1 medium initially until pellets were formed
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