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Furthermore both types of samples exhibit associated fluorescence emission spectra that are rather similar
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Nuclei that are influenced by chemical exchange have been shown to correlate with residues known to be critical for protein interactions and enzymatic activity (39,40, 41,42,43,44)
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Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield
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We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane
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