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rSBA showed no change in either the fluorescence emission profile or the secondary CD profile black jack tribute .
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8 A to those in Fig black jack tribute .
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The concentration of ANS, as determined by absorbance at 370 nm, using ε^sub 370^ = 6800 M^sup -1^Cm^sup -1^ (in methanol), ranged between 10 and 450 µM although in most experiments the upper limit was 320 µM black jack tribute .
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Moreover, the Mg^sup 2+^-dependent docking efficiencies of 2WJ, 3WJ, and 4WJ ribozyme constructs determined by trFRET are generally in accord with the results shown in Fig black jack tribute .
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