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. A significant intensity decrease was observed for most IR bands from native secondary structures, accompanied with the exposure of most Trp residues to water and the formation of aggregates during the I [arrow right] A transition
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. The recomhinant form also shows emission maxima at 327 nm for the native stale and 357 nm in the completely denatured state (Fig
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. Since contact order is well-established as a "single value descriptor of topological complexity" (68)
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. 22-mm thickness were cut from a rolled plain sheet into a rectangular shape (10 mm × 2 mm) and flattened carefully to remove any curvature on the specimen surfaces
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. of Physical Chemistry-Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands
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. Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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. All results were tit to a binding isotherm after correcting for dilution and background scattering
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- It was an audacious goal, and reaching it, according to OFHEO, became Fannie Mae's reason for existence: "$6
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. At the same time, Seniors Coalition, America 21, and 60 Plus inundated seniors with mailings praising his opponent (no groups offset this with support for Feeley)
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. For Trp residues in proteins, it is not uncommon to find average lifetimes that are significantly longer than for free Trp
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. Most intriguingly, Hh has an inverse effect on the development of eyes and taste buds: the smaller the eyes, the more taste buds are produced, which undoubtedly lead to an enhanced sense of taste
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. This clustering suggests a direct relation between the observed conformational exchange and the CaM-SEF2-1mp interactions (vida infra)
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.Binding affinity of ANS, DCVJ, and ThT to early misfolded TTR oligomersSamples containing 1 µM total concentration of misfolded A-state TTR monomers packed into soluble oligomers harvested 2 h after misfolding initiation were used as bait for probe binding
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. High-resolution nuclear magnetic resonance studies of proteins
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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.48), and the anus was situated near the midpoint of the body (Fig
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. CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5
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. Almost all (80 to 90%) occur in persons younger than 19 years of age, and 50% occur in children aged 10 to 14 years
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.* Directed research should be undertaken (or continued) to determine the most effective ways of treating problem gamblers who are women, seniors, youth, young adults, members of ethno-cultural communities and Aboriginals
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