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Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure boat guaranteed loan .
Samples were degassed under vacuum for 10-15 min with gentle stirring before being loaded into the calorimetric cell boat guaranteed loan .
The use of fluorescence methods to monitor unfolding transitions in proteins boat guaranteed loan .
5 ns with S^sup 2^^sub s^ of ~0 boat guaranteed loan .
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We treated Gα^sub s^(GDP) with dithiothreitol (see Philip and Scarlata, 2004) and found that its pressure behavior was unchanged (data not shown) boat guaranteed loan .
The second DSC peak is centered at 84°C, which is similar to the apparent T^sub m^ determined from the peak position in the first derivative of the CD melting data, dΘ^sub 222^(T)/dT (Fig boat guaranteed loan .
1 C was rapidly cooled to 5°C and scanned again from 5 to 100°C boat guaranteed loan .
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1 and then normalized by dividing it by the number of atoms of each residue boat guaranteed loan .
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