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Repulsive interparticle interactions in a denatured protein solution revealed by small angle neutron scattering
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Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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The relatively high drug concentration resulted in noticeable osmotic swelling (up to 6% in diameter, corresponding to a fractional volume change of ~20%) but did not seem to affect the overall state of the oocytes
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The overexpressed dimeric enzyme has four glycosylation sites per monomer, with carbohydrates accounting for ~10% of the dry weight
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Figures 6 and 7 reveal that comparatively NC flux gives lower contact angle values than R flux or WS flux regardless of the solder types and temperatures for Cu substrate
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For k^sup -1^^sub ex^200 µs
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The model used was[pi](FL) = P(Y = 1 | FL) = [e
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. Among the several azurin mutants available, I7S, F110S, and C3A/C26A maintain a native-like fold (19,22) and will therefore present the same surface topology/chemical composition to the ice-water interface
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