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. In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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. The relaxation data were measured at multiple magnetic fields, and analyzed in a model-free manner using in-house written software designed to detect nanosecond internal motion
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. Experimentally these states have been principally observed in colloidal systems although it has been shown that there are strong analogies between the behavior of colloidal and concentrated globular protein solutions (2)
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. The majority of HDL contains both apoA-1 and apoA-2 (LpA-1/A-2); most of the remaining particles contain apoA-1 but not apoA-2 (LpA-1), and a small fraction contains only apoA-2 (LpA-2) (Cheung and Albers, 1984; Pastier et al
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.3 mol of reactive SH group per mol of monomer under native conditions, indicating that one of the thiol groups of GR is partially accessible to DTNB in the folded protein
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