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Native-like mean structure in the unfolded ensemble of small proteins business florida loan small .
As this is not available one can at best calculate the minimum average number of binding sites per macromolecule, n, by assuming the maximum fluorescence intensity increment, ~110-fold, ever observed between free and protein-bound ANS (φ^sub Fprotein^/φ^sub Fice^ = 0 business florida loan small .
When the binding reaches saturation, nonbound molecules dominate the diminazene signal in the nucleus business florida loan small .
In this case, however, stabilization of the native state is most probably due to a decrease in free energy of the native state; phosphate, like sulfate, is a salting-out ion that stabilizes folded protein structures and favors aggregation and precipitation business florida loan small .
Upon peptide binding, the flexibility of side chains located in the binding sites is redistributed business florida loan small .
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