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. For instance, we have shown that the thennodynamic folding parameters (ΔC^sub p^ and m-values) of the HPr of S
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. The shape of the emission spectrum of pure L-tryptophan (see Fig
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. Thus, (τ^sup o^^sub m^)^sub app^ contains information about the orientation of the N-H^sup N^ bond vector via the angle Φ
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. 9 shows the dependence of η^sub r,0^ on BLG concentration at pH 5
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. In comparison, the ThT fluorescence peak in a water-glycerol mixture remains constant at 492 nm
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. The majority of HDL contains both apoA-1 and apoA-2 (LpA-1/A-2); most of the remaining particles contain apoA-1 but not apoA-2 (LpA-1), and a small fraction contains only apoA-2 (LpA-2) (Cheung and Albers, 1984; Pastier et al
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. By using model disks comprised of apoC-1 and DMPC, we showed that the heat-induced protein unfolding is a slow thermodynamically irreversible (or non-equilibrium) transition
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. Nor does it anticipate specifically evolved favorable departures from the random imbalances of entropy and energy assumed here, which certainly arise and roughen the landscape, nor does it account for specific behavior from hydration shells
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. Samples were excited at 270 nm (16-nm bandpasses for excitation and emission)
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. More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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. In contrast, addition of DCVJ and ThT to misfolded aggregates generated from A-state TTR results in a dramatic increase in the quantum yield of the different probes (Fig
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.Φ-Values: normative contributionsAs a second general prediction, and as an example of the specific calculations possible with the model, we examine the important question of Φ-value analysis and its interpretation
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. However, we obtained no useful information on the glass transition temperature T^sub g^
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