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. Interestingly, when GR was incubated in the presence of 3 M GdnHCl the area of the 120 kDa peak decreased markedly, and a second peak corresponding to a molecular weight of ~60 kDa appeared
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. When excited at 280 nm, gSBA shows a fluorescence emission maximum at 328 nm, which is red-shifted to 354 nm on denaturation with 7
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. We followed a three-part approach
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. Evidence of a thermal unfolding dimcric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure
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. 5 mL samples were placed in cylindrical spectrosil cuvettes, 4 mm inner diameter, and rapidly frozen in liquid nitrogen
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. Alterations in ^sup 1^H NMR peak levels from intracellular lipids have been reported in various cell biological processes, such as cell activation, proliferation, and apoptosis (31)
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. GENERAL DISCUSSIONThe compositions at which the relative viscosity (Fig
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. HDL containing both apoA-1 and apoA-2
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. W rangeYearIchthyoplankton surveys1978-79 61[degrees]58'-66[degrees]01'198254[degrees]45'-61[degrees]57'198358[degrees]00'-65[degrees]07'198565[degrees]05'-67[degrees]18'198661[degrees]27'-65[degrees]05'62[degrees]40'-66[degrees]51'199156[degrees]03'-56[degrees]59'199560[degrees]00'-67[degrees]00'199662[degrees]03'-65[degrees]16'199861[degrees]00'-65[degrees]44'199962[degrees]59'-65[degrees]12'200063[degrees]37'-66[degrees]48'200162[degrees]12'-67[degrees]33'62[degrees]30'-66[degrees]12'61[degrees]55'-65[degrees]01'Posttransition juvenile trawls199262[degrees]45'-66[degrees]14'199863[degrees]51'-66[degrees]43'199963[degrees]51'-66[degrees]42'200061[degrees]53'-67[degrees]25'200162[degrees]30'-66[degrees]12'Table 2Body proportions of Pseudopercis semifasciata larvae, according to theflexion stage of the notochord
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. The backbone atoms (N, Cα, and C') of the most stable structural elements of both proteins, the first α-helix of gp120 (residues 17-31), and part of the β-sheet of CD4 consisting of residues 26-30 and 82-86 (see Fig
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. Age truncation, an inevitable result of fishing, can increase recruitment variability by reducing the length of the spawning season or by selectively removing older, more fit individuals from the population
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. [FIGURE 6, 7 & 8 OMITTED]Similar to the ANCOVA results for abso-lute fecundity, there were no differences inslopes or elevations for relative fecundity forprefertilization females for the years 1996-98 (Table 4)
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. 1 Epidermal growth factor receptor is a cell membrane protein, providing signal transduction and cell growth
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.Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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. We attributed this unfolding to cold denaturation of the S-S linked dimer (Gursky and Atkinson, 1996b), yet other studies suggested the importance of the oligomer dissociation (Osborne et al
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.1 nm, in agreement with the size assessed from the native gel (Fig
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. In fact, the 3WJ ribozyme does not fold significantly (to a compact structure) in the absence of loop A, indicating that the loops are more important than the junction for tertiary folding in this case
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