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. In this case, deglycosylation lowers the T^sub m^ of the peptide by 20°C, whereas the free energy of unfolding is reduced by only 1 kcal/mol (26)
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. The lowest protein concentration of 7 µg/ml reveals a large red shift with λ^sub max^ of ~345 nm, whereas protein concentrations of 50 µg/ml and 1 mg/ml result in λ^sub max^-values ~340 nm for the unfolded states
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. In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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.Using CPM-PLCβ as a readout of possible conformational changes corresponding to Gβγ activation, we monitored CPM-PLCβ fluorescence under pressure and compared it directly to using CPM-PLCβ with supersaturating levels of Gβγ-subunits
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. However, a quantitative relation between the topomer search space and contact order is still unclear, since no native contacts are actually required to form at a purely topologically-defined transition state at all (although many are to be expected from the partial ordering at the secondary level at least)
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