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. At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range
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. As shown in Fig
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. In addition, the distance distributions are peaked at larger distance values as sequence separation increases
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. The shape of emission spectra as obtained from GndHCl-induced and thermal unfolded states also reveal some clear differences (Fig
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. Several NMR studies have been carried out on protein-ligand complexes (reviewed in Palmer (5)), suggesting that both increases and decreases in protein entropy may occur upon ligand-binding
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. %) into the SnAgCu solder system reduces the melting temperature from 218°C to 214°C, but the solder joint lifetime is the key issue that determines whether this Pb-free solder is acceptable
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. Although amyloidal isolates either from in vivo samples or in vitro preparations are generally straight, some of them display more or less pronounced curvature (e
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. On average, CD4 shows an intraresidue entropy decrease of -0
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.Finally, given the role of GR in the maintenance of cellular redox state, preservation of a stable enzymatic capacity is likely to be very important for cell survival (under stress situations, in particular)
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. Conversely, P(x) is maximal for small x if one assumes a Flory-style random coil model with uncorrelated fluctuations and uniform (mean-field) chain swelling
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. Changes in the CD signal at 208 nm (where the parallel polarized π-π* transition occurs) are the result of a flexibility of the structure (46)
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. It should be noted that the diminazene aceturate concentration we used for the cellular uptake experiment is relatively high (250 mM) but still not even half a percent of the concentration of water which dominates the MR signal
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.A control comparison of HuBChE-H2O and HuBChE-D^sub 2^O (not shown) revealed identical MSDs below 260 K, but higher MSDs of the H2O-hydrated sample at higher temperatures
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. Its characteristic features are: i), specific high-affinity binding in the presence of fast exchange, ii), accommodation of a relatively nonmatching and flexible target via exchange between multiple binding alternatives, iii) dimerization of CaM upon target binding, and iv), large-scale (domain) motions on the nanosecond and microsecond timescale
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. Cytoplasmic concentrations relevant to macromolecular crowding are typically up to 40% v/v (72)
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. 1 C), yet it shows hysteresis and scan rate effects characteristic of thermodynamically irreversible reactions, as indicated by the CD and light-scattering data in Fig
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. Annual reproductive cycle of oocytes and embryos of yellowtail rockfish, Sebastes flavidus (family Scorpaenidae)
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. At low Mg^sup 2+^, all three ribozymes show a single peak at low E, reflecting an extended, undocked state
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