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This work was supported by a grant from the Dept
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Instead, new local contacts would he added onto those that already exist in the denatured state
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In conclusion, the results from both quenchers suggest that at acidic pH, the aromatic residues became accessible to the solvent, although they were not as exposed as when EI^sup sc^ was fully unfolded (7 M GdmHCl)
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Due to the high sensitivity of tryptophan fluorescence with respect to changes in the microenvironment of the intrinsic chromophores, various properties of the unfolded state can have a distinct effect on the λ^sub max^-values
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