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Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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Observation of an exothermic process associated with the in vitro polymerization of brain tubulin
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Researchers from around the world are working on this concept
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Unlike simple hyperbolic binding curves, profiles such as those of Fig
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The Sf9 expression system allows for postsynthetic modifications of the proteins
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1 nm, in agreement with the size assessed from the native gel (Fig
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However, a quantitative relation between the topomer search space and contact order is still unclear, since no native contacts are actually required to form at a purely topologically-defined transition state at all (although many are to be expected from the partial ordering at the secondary level at least)
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Large enthalpic (-259 ± 13 kJ µmol^sup -1^) and entropic changes (220 ± 13 kJ mol^sup -1^ at 310 K) upon binding were observed experimentally (Kwong et al
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