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Although the glycosylated form undergoes a simple two-state unfolding, the nonglycosylated species unfolds via a compact monomeric intermediate that is not a molten globule
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However, the molar ellipticity at 222 nm at basic pH was larger (in absolute value) than that at pH 7
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3 this behavior is shown for BAA
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To minimize localization errors, an rf bandwidth of 11 kHz was chosen, which required gradients of up to 1
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5 ms for (200 µm)^sup 3^ voxel spectra
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of Physical Chemistry-Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands
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47 ml g^sup -1^, obtained from the R^sub h^ of the BLG monomer in dilute solution, leads to values of φ for the more concentrated solutions which are above the expected φ^sub max^ or, using the reverse of the above conversion, a mass fraction of 43
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The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7
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The misfolding and aggregation reaction as monitored by chemical cross-linking (Fig
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From the fit it was obvious for all probes that there were burst phase responses going from the signal from the monomeric unfolded state (U) to the A-state
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Unlike the majority of interstellar clouds, which serve as nurseries for new stars and measure some 10 to 30 light-years across, most cemeary globules span only about one-tenth that distance and lack sufficient gas and dust to form stars
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The results of ANCOVA (Table 4) over a common age range showed no evidence of differences in slopes among the years 1996-98 (P=0
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