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. Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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. Two-dimensional infrared correlation (2D IR) analysis was carried out using SDIAPP software developed in-house (27) according to the generalized two-dimensional (2D) correlation algorithm (29)
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. For example, scaling of chain size with chain length can provide a direct probe of the nature of chain-solvent interactions (37,38,42,44,45)
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. Many of these have been considered in greater detail in previous columns
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. "Molten-globule" state accumulates in carbonic anhydrase folding
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.During the wetting balance tests, Cu atoms diffuse from the substrate into the solder, while the Cu substrate is immersed into the molten solder bath
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