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. For information about the CDRF and the research it supports, visit www
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. Conversely, at acidic pH, the percentage of β-turns appearing at 1668 cm^sup -1^ was decreased, and the percentages of helical structure, loops, and disordered structures (bands at 1648 and 1657 cm^sup -1^, which could not be independently assigned at pH 3) were higher (50
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. For most proteins, which typically bear more than one tryptophan, the observed emission spectrum is composed of multiple contributions of the five spectral classes
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. As this is not available one can at best calculate the minimum average number of binding sites per macromolecule, n, by assuming the maximum fluorescence intensity increment, ~110-fold, ever observed between free and protein-bound ANS (φ^sub Fprotein^/φ^sub Fice^ = 0
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. This same volume change is obtained for a peptide derived from Gβ which also activates PLCβ
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. For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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. Also plotted in Fig
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