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The acquisition parameters were TR = 2000 ms, SW = 10 kHz, acquisition size = 4096, NEX = 256, T^sub acq^ = 8
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This is not, of course, to suggest that the path is unique, since: 1), a large fraction of each subdimension may be explored; 2), the path is at each stage reversible; 3), degrees of freedom inessential to the current dimensional reduction are not "frozen" but are freely exploring their configurations; and 4), the nondiffusive "zipper" dimensions describing the local folding of secondary structure are perpetually exploring their own configurational space rapidly and cooperatively with the slow dimensions
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Clearly, the four-way helical junction in the native ribozyme is the most important determinant for proper folding, guiding the approach of arms A and B
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