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. These difference maps are shown in Fig
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. However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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. Even for TAKA, which does not show any aggregation, we observed slightly smaller red shift for thermal unfolding, but due to missing aggregation still larger as compared to BLA, BAA, and BSUA
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. For magic angle spinning (MAS) experiments, an indirect detection pulsed field gradient nano H{X} probe (4 mm) was used in a Varian (Palo Alto, CA) UNITY INOVA 500 NMR Spectrometer
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