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Three pHs were chosen based on the steady-state fluorescence results: pH 5, close to the midpoint of the titration observed in ANS-binding and fluorescence intrinsic experiments; neutral pH, where the intensity of steady-state fluorescence experiments was at its maximum; and pH 12, in the basic region graduate loan private student .
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In contrast all thermal unfolded states (with the exception of those obtained from PPA) exhibit λ^sub max^ values around 340 nm, which correspond to much smaller red shifts upon unfolding graduate loan private student .
The initial decrease in fluorescence intensity takes place despite the solidification of water raised by ~55-fold the concentration of ANS in the liquid water pool of ice (12) graduate loan private student .
As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered graduate loan private student .
Strambini, unpublished data) provides additional evidence that the creation of these low affinity ANS binding sites is owed to perturbations of the protein fold associated with the frozen medium graduate loan private student .
In a second experiment, we compared dimeric DmAChE and tetrameric HuBChE graduate loan private student .
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Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments graduate loan private student .
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