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. In this work, we show that we can simulate conformational ensembles, with atomistic detail, such that ensemble characteristics match the predictions for polymers in the EV limit
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. org and during the 3rd International Symposium on Milk Genomics & Human Health, which will be held September 19-21, 2006, in Brussels, Belgium
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. Interdomain interaction and substrate coupling effects of dimerizalion and conformational stability of Enzyme I of the Esclierichia coli phosphoenolpyruvate:sugar phosphotransferase system
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. The first step in sugar transport: crystal structure of the amino terminal domain of enzyme I of the E
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. Delivery of drugs across cellular membranes is one of the main goals of current pharmaceutical research (43), including MRI contrast agent development for biological applications (44)
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. A 45% decrease in activity was recorded after the neutron scattering experiment
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. In the same study we could also conclude that SEF2-1mp lacks any well-defined secondary structure when interacting with CaM, an observation very unusual for CaM-bound peptides
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. We have developed a method for analyzing ^sup 15^N-spin relaxation measured at two magnetic fields that corrects (τ^sup B^^sub m^)^sub app^ for internal motion up to ~4 ns, giving (τ^sup o^^sub m^)^sub app-corr^ (22)
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. Another example of this projection is found in the master-equation approach (Zwanzig, 1995), in which the smoothly funneled high-dimensional energy landscape implies some shaping of the nonnative contacts of the underlying model
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. But is coolant as essential to every machining process as it is to a car?The cost of coolant is approximately 15 percent of the life-cycle operational cost of a machining process
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. The period of parturition as indicated by the occurrence of ovaries containing eyed larvae extended from the second week in January through the second week of March
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. The distance dependence of the FRET efficiency provides a very sensitive fluorescence-based measure of distances in the 20-80 [Angstrom] range
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. Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17)
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. This collective process could explain the fact that essentially all resonances affected by conformational exchange have approximately the same time constant and are clustered in regions important for either CaM:CaM or CaM:SEF2-1mp interactions
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. In the solutions containing 100 mM NaCl and 10 mM acetate buffer alone, this interaction becomes increasingly attractive at short range
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