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In EI^sup ec^, the thermal midpoint of the transition monitored by fluorescence is concentration-dependent (52), but the slopes of the native baseline are smaller due to the fact that two tryptophan residues are observed in the C-terminus of EI^sup ec^
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Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig
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