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Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH jacksonville used car loan .
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Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig jacksonville used car loan .
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