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. For indole in aqueous solution, k^sub q^ for iodide quenching is 6
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. Equation 2 implies that when contact angle θ^sub c^ increases, the surface tension force moves toward negatives due to the cosine value of θ^sub c^, resulting in the negative wetting force
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. 4, there is a good correlation between the chemical potentials of monocations in solutions of PEG with different molecular weights
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. Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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. Partitioning the configurational space in this way, and by providing an attractive "gutter", relying on local forces alone, to connect one diffusive subspace to the next, creates the advantageous funnel landscape when the high-dimensional structure is projected into a one- or two-dimensional space
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. These may or may not be identified as intermediate states, depending on their occupancy lifetime
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. Conjugated linoleic acid (CLA) is another component found only in ruminant products (like cheese)
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. Additionally, only this ribozyme is almost completely docked at physiological concentrations of Mg^sup 2+^ (~2 mM)
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. This level of proliferation is broadly similar to that reported previously for primary human chondrocytes cultured in alginate beads over a four-week period
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. 1 A shows fluorescence emission spectra of GR in the absence or in the presence of GdnHCl
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. The phase diagram analysis, which is a sensitive tool to detect folding intermediates (35), was performed to characterize whether an intermediate was involved in ACYI thermal unfolding
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. Selective binding and solvent denaturation
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.Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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. Karplus and Weaver (1994), for example, shows a fast folding route of hen lysozyme in which the early formation of β-sheet structure permits the final approach to the native state to proceed in a subspace of reduced dimension
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. In the hypergutter picture, the nine-dimensional search space of these critical regions separately is reduced to three sequential three-dimensional searches by the long lifetimes of the native regions when the remainder of the protein is disordered
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. However, when a polymer-solvent system undergoes LLPS prior to crystallization in a certain range of polymer concentration (see Figure 1), the crystallization curve is modified
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. In this case, the apparent crystallization temperature is less than the equilibrium melting temperature, and the monotectic point shifts slightly toward higher polymer concentrations as the cooling rate increases [10]
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