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. However, it has been shown in various cases that (and how) under specific conditions λ^sub max^-signals can be used for quantitative analyses of unfolding transitions (4,12,15)
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. That a two-contribution model is needed follows from a comparison of average experimental and theoretical R^sub 1^ values
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. The quenched Trp fluorescence in the presence of ANS and Bis-ANS (Fig
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. A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. If one were to examine chain properties at length scales that go above l^sub s^, properties of denatured proteins for different sequences should become indistinguishable from each other
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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.In this context we wish to stress that, since IENS measures global mean atomic fluctuations (i
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.DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCβ-Gβγ interactions on membrane surfaces and in buffer
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