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. Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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. Samples were excited at 270 nm (16-nm bandpasses for excitation and emission)
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.6^, δ) for Fyn SH3 domain
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. Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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. The ratio of R^sup 2^ over R^sup 1^ (R^sup 2^ corrected for R^sub ex^; vide infra) at a magnetic field B gives the apparent correlation time, (τ^sup B^^sub m^)^sub app^ (Eq
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. This agrees with the DSC data from an identical sample indicating that disk reconstitution after heat denaturation takes more than 48 h to complete at 24°C (Fig
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. This broadening/tailing is evidence for occupation of two (or more) structures that are interconverting on a timescale similar to or slower than the average burst duration
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