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. Between 2 and 5 M GdnHCl, a significant drop in anisotropy was observed, indicating faster rotational mobility of tryptophan residues upon unfolding of GR
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. This energy difference is used with the Metropolis criterion (61) to accept or reject the proposed move
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. ANS binds to a small extent to the unfolded monomeric form of TTR (Table 2), providing an excellent reference point for molecular dynamics studies of the quaternary structure of TTR
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. It is noteworthy that all G-protein subunits are posttranslationally modified with hydrocarbon chains (see Wedegaertner et al
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