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The conformational stability and structure of the EI from Streptomyces coelicolor, EI^sup SC^, were explored in the absence and in the presence of its effectors by using several biophysical probes (namely, fluorescence, far-ultraviolet circular dichroism, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry) and computational approaches
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The initial decrease in fluorescence intensity takes place despite the solidification of water raised by ~55-fold the concentration of ANS in the liquid water pool of ice (12)
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R^sup 1^ and R^sup 2^ values were determined by fitting peak volumes to a two-parameter single exponential decay using MATLAB
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Here, we have used fluorescence methods to follow the membrane association of a chimeric PLCβ/δ, referred to here simply as PLCβ (see Methods) under pressure
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To determine the activation energy (enthalpy) E^sub a^ [congruent with] ΔH*, the Θ^sub 222^(t) data were fitted by exponentials (Fig
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Karplus and Weaver (1994), for example, shows a fast folding route of hen lysozyme in which the early formation of β-sheet structure permits the final approach to the native state to proceed in a subspace of reduced dimension
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Alternatively, our picture suggests ways of increasing folding times greatly by selective mutations that retain topology and stability of the native state, but destabilize one or more of the on-pathway diffusional subspaces so that intermediate searches are required in d > 3
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brasilianus was found by itself only at three stations
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For further simulation details, we refer to Hsu and Bonvin (2004)
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For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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