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1628 cm^sup -1^ or the fluorescence intensity at 320 nm vs
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Small Angle X-Ray Scattering
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Furthermore, studies with solubilizing agents such as detergents, reversed micelles, or nondetergent sulfobetaines (NDSB) proved that aggregation, which typically occurs during thermal unfolding in pure buffers, is strongly suppressed when such agents are added to the solution (29,32)
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Finally, they were cleaned by ethanol and dried properly to remove any contamination
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Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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Address reprint requests to Suzanne Scarlata, E-mail: Suzanne
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4 consists of four cordlike subunits
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Early life history of the Argentine sandperch Pseudopercis semifasciataoff northern PatagoniaLeonardo A
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. Thus, the value for K^sub SV^ will depend on the particular average intrinsic fluorescence lifetime of the protein, and may be higher than the value for free Trp
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