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Thus the intrinsic Trp fluorescence was used to investigate the changes of the tertiary structure of ACYI during thermal denaturation
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Lower concentration rates of protein to trypsin were used (100:1, 200:1, and 300:1), but in all these experiments the amount of nondigested EI^sup sc^ in the absence of effectors was insignificant after 1 min of reaction (data not shown)
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CONCLUSION It has been demonstrated in this study that the λ^sub max^-values of tryptophan fluorescence emission are meaningful parameter to characterize properties of the unfolded state
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Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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Rheology of monodisperse latices
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Address reprint requests to Per Hammarstrom
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Here, we have used fluorescence methods to follow the membrane association of a chimeric PLCβ/δ, referred to here simply as PLCβ (see Methods) under pressure
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Furthermore, the cooling curve of the apoA-2:DMPC sample shows partial protein refolding below 80°C, followed by additional refolding between 32 and 24°C, i
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The C^sub p^(T) data of intact disks shows two peaks (Fig
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The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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This permitted sufficiently accurate height determination (maximal vertical resolution was 0
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23 kJ K^sup -1^ mol^sup -1^
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1% of the males reporting that they had compared to 66
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. General description of dielectric spectroscopy This work was supported by Ministry of Education and Science, grant number 1167203 and CIRTU
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. The results are shown in Fig
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