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. Sales of amphetamines like Adderall rose more than 2,000 percent in that time
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. Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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. They are active as dimers, where the two basic regions bind DNA symmetrically as α-helices on opposite sides in the major groove
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. Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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. To determine whether this could be the case for PLCβ, we labeled the enzyme with CPM
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. Rather than invoking a continuous energy landscape with long-range forces giving rise to a spatially funneled landscape, we use rather general considerations to point to a high-dimensional structure of "hypergutters"
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. It is clearer in experiment than the subsequent dimensional reductions because it is the only one that makes significant changes to the radius of gyration of the protein
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. ) Encouraged by the rough agreement between the theoretical and experimental binding enthalpies, we aimed at extracting the binding entropy from our simulations and at gaining insight into CD4 binding-induced conformational changes in gp120 that are related to entropy changes
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. These fundamental aspects of RNA folding are frequently obscured by the strong tendency of RNA to form alternate secondary or tertiary structures, which act as kinetic traps and retard the overall rate of folding
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. In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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. NATIONAL SCIENCE EDUCATION STANDARDSLife Science; Science in Personal and Social PerspectiveLESSON STRATEGYIntroducing the Topic* Before the lesson begins, hold a class discussion based on these questions: What is a prescription drug? What does it mean to abuse a prescription drag? Is it ever OK to take a prescription drug that was not prescribed specifically for you? Can prescription drugs be as dangerous as illegal drugs?* Tell students that they are going to see how much they know about prescription drug abuse and what the latest research is teaching us about it
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. MethodParticipantsOne hundred fifty-two students (76 men and 76 women) enrolled in introductory psychology courses at a small Midwestern university were recruited to participate in the current study
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. Here, we detected a maximum of ~0
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. The gyration tensor is computed as an outer product of the radius of gyration vector
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. Evolutionary conservation and variation of protein folding pathways
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. Janssen; unpublished data; March 2006)
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. These azurins maintain an intact native fold (x-ray structure) but are ~6 kcal/mole less stable relative to the wild-type (WT) (18-22)
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. It was pointed out that this trend probably reflects multiple binding sites of varying affinity, likely a consequence of the broad distribution of protein conformations in ice (3)
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.* Chain drug stores now account for 52 percent of all drug stores, up from 37 percent in 1991, and they employ some 560,000 people at more than 20,000 locations
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