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. For lipid T^sub 2^ measurements in the cytoplasm, localized spectroscopy with (200 µm)^sup 3^ voxels was employed with 10 different echo times in intervals of 4 ms, starting at 6
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. For small globular proteins, such as BLG, the volume of this layer is comparable to the volume of the dry protein
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. Taken together, these data indicate that heating of the apoA-2:DMPC disks to 99°C leads to protein unfolding and disk-to-vesicle fusion; consecutive cooling leads to a gradual reduction in the vesicle size and partial protein refolding, followed by formation of smaller protein-containing vesicles and additional helical refolding at 34-24°C
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. Arrhenius plots for these phases are linear and intercept near 100°C (Fig
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. For example, a nonnative attraction beween the helices irrespective of their axial orientation would favor a two-dimensional search out of contact before a three-dimensional search in contact as the structure of the five-dimensional search space
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