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In brief, the intrinsic Trp fluorescence emission spectra were measured using a Hitachi F-2500 spectrofluoromeler (Tokyo, Japan) using 1-cm path-length cuvettes with an excitation wavelength of 295 nm missouri used car loan .
Spectroscopic methods for analysis of protein secondary structure missouri used car loan .
, the average overall 10^sup 4^ conformations, was used to compute the average Kratky profile missouri used car loan .
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Protein dynamics: comparison of simulations with inelastic neutron scattering experiments missouri used car loan .
Thus, the data are consistent with a motional model in which the two N-terminal and two C-terminal CaM domains in the dimeric CaM:SEF2-lmp complex undergo a small-scale wobbling motion with a half-angle of ~20° as estimated from S^sup 2^^sub s^ = 0 missouri used car loan .
Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes missouri used car loan .
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Slowing down the scan rate also leads to a reduction in the CD amplitude in the cooling curves below 80°C (Fig missouri used car loan .
In summary, our comparative study of different ribozyme constructs shows that in contrast with the 2WJ and the 3WJ, the natural 4WJ architecture can efficiently guide conformational search events during ribozyme folding, thereby dramatically influencing subsequent tertiary structure formation and stability missouri used car loan .
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