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. Unfolding was monitored by a variety of techniques, including intrinsic fluorescence emission, anisotropy and iodide quenching measurements, far-ultraviolet circular dichroism and thiol reactivity measurements
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. Third, in contrast to the reversible unfolding of lipid-free protein (indicated by the agreement between the heating and cooling CD curves in Fig
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. Appending the complex trajectory of gp120 to the free one gives rise to a small increase (ΔS^sup 2nd,f+c^^sub CD4,F43^ = 0
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.1] = regression coefficients for the intercept and fork length, respectively
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